Bukit Vatican merupakan nama yang diberikan kepada salah satu bukit yang berada di tepi Sungai Tiber bertentangan dengan ketujuh-tujuh bukit Rom tradisional. Bukit ini dinamakan jauh sebelum permulaan agama Kristian. Bukit ini mungkin merupakan tapak sebuah bandar pendudukan orang Etruria yang dipanggil Vaticum. In the 1st century A.D., the Vatican Hill was outside the city limits and so could feature a circus and a cemetery. St. Peter's Basilica is built over this cemetery, the traditional site of St. Peter the Apostle's grave. There was another cemetery nearby, which was opened to the public on 10 October 2006, to commemorate the 500th anniversary of the Vatican Museums.